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Synthesis of core-shell nanocrystalline cadmium diselenide/cadmium sulfide and determination of lysozyme by fluorescence method

wallpapers News 2021-11-23
Synthesis of core-shell nanocrystalline cadmium diselenide/cadmium sulfide and determination of lysozyme by fluorescence method
Cadmium di-selenide/cadmium sulfide core/shell quantum dots (QDs) were synthesized and modified by 3-mercaptopropionic acid (MPA) in an aqueous solution. In contrast with conventional organic dyes, these QDs are brighter, more stable against photobleaching, and do not suffer blinking. The fluorescence intensity of these QDs is also more stable than MPA-capped CdS nanocrystals. The applicability of the results of these QDs to the determination of lysozyme was studied successfully. The pH value was selected at pH 7.40, with excitation and emission wavelength at 365 nm and 642 nm, respectively. Under optimal conditions, a linear relationship was found between the relative fluorescence intensity and the concentration in the range of 0.5 similar to 8.0 mg/L and 8.0 similar to 32.2 mg/L for lysozyme (Lys), and the linear equation was Delta F = 0.97 + 2.21 C (mg/L) and Delta F = 12. 20 + 0. 70C (mg/L), respectively. The detection limit defined as three times the standard deviation of the blank(3 sigma/K), of lysozyme is 0. 20 mg/L. And real samples were analyzed satisfactorily.
With the improvement of semiconductor nanocrystal synthesis technology and stability, semiconductor nanocrystals have excellent optical properties relative to dyes, and the prospect of possible application in biological detection has attracted widespread attention. To realize the development of fluorescent nanoparticles in biological detection Practical, people are committed to developing effective preparation methods and surface modification technologies for the synthesis of high quantum yield nanoparticles. The main research contents of this article are as follows: 1. Using colloidal chemistry, L-cysteine (L-cysteine, referred to as L-cysteine) -Cys) as stabilizers, prepared CdSe and CdSe/CdS water-soluble nanocrystals.
The potential application value of nanoprobes with excellent optical properties in life sciences and other research has attracted great attention from scientists. As a fluorescent probe, compared with traditional organic fluorescent dyes, its fluorescence spectrum peaks are narrow and symmetric Good performance, strong light resistance, more sensitive detection, and simple operation. In this paper, 11-mercaptoalkanoic acid [HS-(CH2)10-COOH] is used as a modifier to synthesize good water solubility and biocompatibility.
For more than thirty years, solution semiconductor nanomaterials have been widely studied due to their size-related optical properties and the process of solution-phase synthesis. Because of the quantum confinement effect, colloidal semiconductor nanocrystals are also called quantum dots. The particle size of quantum dots is closely related to ultraviolet-visible light absorption spectroscopy (UV-Vis) and fluorescence emission spectroscopy (Photoluminescence), which makes spectroscopy a simple and very effective method for studying quantum dots. Secondly, quantum dots have excellent optical properties. Compared with traditional fluorescent dyes, their fluorescence emission peak position can be continuously adjusted, the full width at half maximum (FWHM) of the fluorescence emission peak is narrow, and the fluorescence quantum efficiency (Photoluminescence Quantum Yield, PLQY) high, high chemical, and photochemical stability. These unique properties make quantum dots have great prospects in the fields of photoelectric-electro-optical conversion, fluorescence display, bioluminescence labeling, and sensors. Cadmium selenide (CdSe) quantum dots have become a classic model system in the field of quantum dot synthesis and application due to their relatively mature synthesis method and emission spectrum close to covering the full visible light region. At room temperature, the bulk crystals of CdSe appear in two different lattices, namely wurtzite (hexagonal lattice) and zinc-blende (zinc-blende, face-centered cubic lattice) structures. However, the research on the crystal form control of CdSe quantum dots has always been a challenging topic in this field.
 

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